Isolation of TEM beta-lactamase gene in Pseudomonas aeruginosa and Imipenem Effect on Expression of TEM Gene by Real-Time PCR from Burn Wound Samples

Background & Aim: Pseudomonas aeruginosa strains that were resistance to majority of commonly used antibiotics were caused problem in treatment of these infections. Imipenem is the excessive potential antibiotic for elimination of antibiotic resistance isolates of these bacteria. Aim of this study was, identification of imipenem effect on TEM beta-lactamase gene expression in resistant to antibiotic, Pseudomonas aeruginosa strains, from burn wound samples, by Real-time PCR. Methods: In descriptive cross-sectional study, 100 burn wound samples were collected from Tehran hospitals. Pseudomonas aeruginosa isolates, were found from samples. After DNA extraction by PCR reaction, were isolated TEM gene from samples contained this bacteria, and were defined imipenem MIC measure, for them. Then, were compared, TEM beta-lactamase gene expression, among imipenem-treated and untreated Pseudomonas aeruginosa strains, by Real-time PCR Results: From 100 wound samples, 60 isolates (60%) were infected by Pseudomonas aeruginosa and in 34 samples (56.6%) of Pseudomonas aeruginosa isolated, were presence TEM beta-lactamase gene. Expression of TEM gene, 78.68% decreased in imipenem-treated Pseudomonas aeruginosa strains, compare by untreated strains. Conclusion: According of this study finding, from imipenem by anti-beta-lactamase action that was interested on reduction of TEM gene expression, could be used in control of antibiotic resistance Pseudomonas aeruginosa strains.